Jessica+Sima-+log

I would like to write my paper on the LC-MS/MS methods for analyzing testosterone levels

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 * [remove span tags and link to article JCB]**

[|**Validation of bioanalytical LC–MS/MS assays: Evaluation of matrix effects**]
 * [Full Marks JCB]**

//Ann Van Eeckhaut, Katrien Lanckmans1, Sophie Sarre, Ilse Smolders, Yvette Michotte∗//

//Vrije Universiteit Brussel, Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information, Laarbeeklaan 103, 1090 Brussels, Belgium//


 * Looks at the matrix effect on LC-MS/MS methods of testosterone analysis to better understand how matrix effect might compromise integrity of the test
 * Also compares ESI techniques to APCI techniques
 * Competition for molecules to turn into the gas phase when using APCI but not ESI
 * Use of post-column infusion and post-extraction spike method to compare the matrix effects between the two modes of ionization
 * Suggests methods to overcome matrix effects
 * Sample prep techniques: diluting the original sample or injections of smaller volumes, protein precipitation for sample cleanup, solid phase extraction, liquid-liquid extraction to purify sample
 * Chromatographic methods: gradient elution, HILIC methods
 * Mass spec conditions: APCI or ESI ionization
 * Internal standard choice: shouldn’t compete with the elution of compounds to be analyzed


 * No matter what techniques are chosen, when determining levels of compound from human samples, matrix effect must always be taken into account
 * Adequate sample prep must be taken to remove any competing proteins or compounds
 * The right column type must be chosen for the analyte
 * APCI ionization is the gold standard

__**FAQ**__

10. Give 5 examples of web resources used to teach spectroscopy to high school students.
[|SpectraSchool] A site hosted by the University of Leicester and the Royal Society of Chemistry that provides a range of resources to help in the understanding of the principles and practices of spectroscopy. The website provides a SpectraGraph program hat enable the user to see the various spectra for a list of certain compounds. Videos and teaching resources can also be found on the site.

[|Squiggly Lines] This website provides links to aid in the teaching and understanding of light and astronomical spectroscopy at the middle and high school level. The site is broken down by topic, first discussing astronomical distances, then the basics of light, and then finally reaching spectroscopy.

[|Mysteries of Science] This website is a collection of links to teaching resources provided by various universities as well as NASA which were all compiled by the SETI Institute. The links range from reading to activities to tutorials. Topics include using a spectrometer, building a spectrometer, and spectroscopy.

[|Cell Phone Spectrometer] Alexander Scheeline at the University of Illinois developed software that can turn any cell phone into a spectrometer. The website provides both instructions and resources for both teacher and student. The site links to the paper about how the software works and allows for free download of the software.

[|The Science of Spectroscopy] This is a wiki-based site that provides introductory material for advanced high school students. The website provides real world application for the involvement of students and external links for further information. //(completed Jessica Sima)//